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Registros recuperados : 73 | |
6. | | MENNA, P.; BARCELLOS, F. G.; HUNGRIA, M. Phylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnll, recA, atpD and dnaK genes. International Journal of Systematic and Evolutionary Microbiology, Great Britain, v. 59, n. 12, p. 2934-2950, dez. 2009. Biblioteca(s): Embrapa Soja. |
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8. | | BORTOLAN, S.; BARCELLOS, F. G.; MARCELINO, F. C.; HUNGRIA, M. Expressão dos genes nodC, nodW e nopP em Bradyrhizobium japonicum estirpe CPAC 15 avaliada por RT-qPCR. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 44, n. 11, p. 1491-1498, nov. 2009. Biblioteca(s): Embrapa Soja. |
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11. | | MENNA, P.; BARCELLOS, F. G.; BANGEL, E. V.; HUNGRIA, M. Taxonomia das estirpes de rizóbios recomendadas para o uso em inoculantes comerciais no Brasil. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIANOS DE INTERESSE AGRÍCOLA (RELARE), 13., 2006, Londrina. Anais... Londrina: Embrapa Soja, 2007. p. 53. (Embrapa Soja. Documentos, 290). Organizado por Rubens José Campo, Mariângela Hungria. Biblioteca(s): Embrapa Soja. |
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14. | | BINDE, D. R.; MENNA, P.; BANGEL, E. V.; BARCELLOS, F. G.; HUNGRIA, M. Análise filogenética com base no gene ribossomal 16S, de 54 estirpes elite de rizóbios utilizadas em inoculantes comerciais brasileiras. In: RELARE, 14., 2008, Bonito. Programa e resumos. [S.l.]: Embrapa Agropecuária Oeste, 2008. p. 13. Biblioteca(s): Embrapa Soja. |
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15. | | BINDE, D. R.; MENNA, P.; BANGEL, E. V.; BARCELLOS, F. G.; HUNGRIA, M. Análise filogenética com base no gene ribossomal 16S, de 54 estirpes elite de rizóbios utilizadas em inoculantes comerciais brasileiros. In: REUNIÃO DA REDE DE LABORATÓRIOS PARA RECOMENDAÇÃO, PADRONIZAÇÃO E DIFUSÃO DE TECNOLOGIA DE INOCULANTES MICROBIOLÓGICOS DE INTERESSE AGRÍCOLA, 14., 2008, Bonito. Anais... Dourados: Embrapa Agropecuária Oeste, 2010. p. 39-40. RELARE. Organizado por Fábio M. Mercante, Oscar F. de Lima Filho, Suelma P. da S. Bonatto. Biblioteca(s): Embrapa Soja. |
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17. | | BATISTA, J. S. da S.; BARCELLOS, F. G.; MENNA, P.; BALLATI, P. A.; HUNGRIA, M. Confirmação da transferência horizontal de genes simbióticos entre gêneros distintos de rizóbios pela caracterização da estirpe de Sinorhizobium fredii CPAC 402, isolada de nódulo de soja nos cerrados. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira : livro de resumos... Gramado: UFRGS: SBCS, 2007. 1 CD-ROM. Pdf. 7324-1775. Biblioteca(s): Embrapa Soja. |
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18. | | MENNA, P.; BARCELLOS, F. G.; BATISTA, J. S. da S.; BANGEL, E.; HUNGRIA, M. Classificação taxonômica, com base no gene ribossomal 16S, de uma coleção de rizóbios recomendados para o uso em inoculantes comerciais no Brasil. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82). Biblioteca(s): Embrapa Soja. |
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20. | | BATISTA, J. S. da S.; BARCELLOS, F. G.; MENNA, P.; BALLATI, P. A.; HUNGRIA, M. Caracterização da estirpe de Sinorhizobium fredii CPAC 402: provável evento de transferência lateral de genes simbióticos em solos dos Cerrados entre gêneros distintos de rizóbios. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2., 2006, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2006. p. 132-138. (Embrapa Soja. Documentos, 276). Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 73 | |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
10/02/2010 |
Data da última atualização: |
03/08/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
MENNA, P.; BARCELLOS, F. G.; HUNGRIA, M. |
Afiliação: |
PÂMELA MENNA, CNPSo/UEL/CNPq-MCT; FERNANDO GOMES BARCELLOS, CNPSo/CNPq-MCT; MARIANGELA HUNGRIA DA CUNHA, CNPSo. |
Título: |
Phylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnll, recA, atpD and dnaK genes. |
Ano de publicação: |
2009 |
Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, Great Britain, v. 59, n. 12, p. 2934-2950, dez. 2009. |
DOI: |
10.1099/ijs.0.009779-0 |
Idioma: |
Inglês |
Conteúdo: |
The genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed when the atpD gene was amplified, and five strains related to B. elkanii revealed a level of variability never reported before. Another important observation was that a group composed of strains USDA 110, SEMIA 5080 and SEMIA 6059, all isolated from soybean, clustered in all six trees with high bootstrap support and were quite distinct from the clusters that included B. japonicum USDA 6T. The results confirm that MLSA is a rapid and reliable way of providing information on phylogenetic relationships and of identifying rhizobial strains potentially representative of novel species. MenosThe genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed... Mostrar Tudo |
Thesagro: |
Fixação de nitrogênio; Rhizobium. |
Thesaurus NAL: |
Nitrogen fixation. |
Categoria do assunto: |
V Taxonomia de Organismos |
Marc: |
LEADER 02813naa a2200193 a 4500 001 1657500 005 2017-08-03 008 2009 bl uuuu u00u1 u #d 024 7 $a10.1099/ijs.0.009779-0$2DOI 100 1 $aMENNA, P. 245 $aPhylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnll, recA, atpD and dnaK genes. 260 $c2009 520 $aThe genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed when the atpD gene was amplified, and five strains related to B. elkanii revealed a level of variability never reported before. Another important observation was that a group composed of strains USDA 110, SEMIA 5080 and SEMIA 6059, all isolated from soybean, clustered in all six trees with high bootstrap support and were quite distinct from the clusters that included B. japonicum USDA 6T. The results confirm that MLSA is a rapid and reliable way of providing information on phylogenetic relationships and of identifying rhizobial strains potentially representative of novel species. 650 $aNitrogen fixation 650 $aFixação de nitrogênio 650 $aRhizobium 700 1 $aBARCELLOS, F. G. 700 1 $aHUNGRIA, M. 773 $tInternational Journal of Systematic and Evolutionary Microbiology, Great Britain$gv. 59, n. 12, p. 2934-2950, dez. 2009.
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